Anti-Mouse IgG (Fab specific) F(ab′)2 fragment antibody produced in goat
SIGMA/M0659 - affinity isolated antibody
Synonym: Anti-Mouse Fab Fragment; Fab Specific Detection; Goat Anti-Mouse IgG Fab
MDL Number: MFCD00162619
Product Type: Chemical
ELISA 10 μg/mL Anti-Mouse IgG (Fab specific) F(ab′)2 fragment antibody produced in Goat (Cat. No. M0659), was probed to detect Mouse IgG by indirect ELISA. Reactivity is observed with Mouse IgG and Mouse Fab but not with Mouse Fc, Human IgG, Human IgA, Human IgM, Bovine IgG, Bovine IgM and Horse IgG.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 2.0 mg/mL |
| conjugate | unconjugated |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| Application: | Anti-Mouse IgG (Fab specific) F(ab′)2 fragment antibody is suitable for use in ELISA (using a conc. Of 2μg/mI (100 μg/well) in 50 mM carbonate/bicarbonate buffer, at pH9.6) . The antibody can also be used in immunoelectrophoresis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice Anti-Mouse IgG (Fab specific) F(ab′)2 fragment antibody is monospecific as determined by immunoelectrophoresis versus normal mouse serum, mouse IgG and the Fab fragment of mouse IgG. The antibody does not react with the Fc fragment of mouse IgG. Moreover, no reactivity is observed with human κ or lambda light chain, IgG, IgA, IgM, IgD and IgE, bovine IgG and IgM, or horse IgG as determined by ELISA. In immunoelectrophoresis, the antibody preparation is found to consist only of the F(ab′)2 fragment of goat IgG, where specific antisera is applied to goat IgG fragments. No contamination with goat IgG whole molecule is observed. |
| Immunogen: | Mouse IgG |
| Other Notes: | Antibody adsorbed with bovine, equine and human serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |