Anti-Myosin VI antibody, Mouse monoclonal
SIGMA/M0691 - ~1 mg/mL, clone MUD-19, purified from hybridoma cell culture
Synonym: Anti-DFNA22; Anti-DFNB37
MDL Number: MFCD02263784
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-Myosin VI Clone: MUD-19 (Cat. No. M0691). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. A431 2. MCF-7 3. MDA-MB-231
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MUD-19, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~150 kDa by SDS-PAGE |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 2 μg/mL using A431 total cell extract | |
| UniProt accession no. | Q9UM54  |
| Application: | Monoclonal Anti-Myosin VI antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunoprecipitation • western blot assay • immunohistochemistry |
| Biochem/physiol Actions: | Monoclonal Anti-Myosin VI antibody is specific for myosin VI in rats, humans and mice. |
| Biochem/physiol Actions: | Myosin VI is involved in the generation of cell shape change, cell motility, membrane remodeling and possibly in organelle and particle transport or tethering. It is also involved in membrane trafficking pathways in cultured mammalian cells where it is associated with the membrane ruffles and the trans-Golgi network. Myosin VI plays an essential role in the development of the mouse inner ear where it is expressed in the hair cell body, in the actin-rich cuticular plate at the base of the stereocilia and in the peri cuticular necklace region. Myosin VI gene mutations are involved in deafness and balance defects in the Snell′s Waltzer mouse. Myosin VI is present in the Golgi complex and may regulate vesicular transport across the membranes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Myosin VI (mouse IgG1 isotype) is derived from the MUD-19 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide corresponding to amino acids of human myosin VI. Myosin VI is a relatively abundant widespread unconventional myosin composed of an N-terminal motor domain, a light chain binding neck region, a coil-coiled region and a highly conserved C-terminal domain. |
| General description: | Myosins are actin-dependent motors that have 15 classes based on their phylogenetics . Myosin VI is an unconventional actin motor that moves towards the minus ends of actin filaments, which is opposite to the movement of conventional myosins. |
| Physical form: | Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |