Anti-MNK2 (N-terminal) antibody produced in rabbit
SIGMA/M0696 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-GPRK7; Anti-MAP kinase interacting serine/threonine kinase 2; Anti-MKNK2
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Immunofluorescence. Antibody recognition: Anti-MNK2 (N-terminal) antibody produced in Rabbit (Cat. No. M0696) is confirmed by immunofluorescence in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing MNK2 (Picture A, B) while no specific staining is detected in the wild type sample-HEK-293T (Picture C, D). Pictures B and D: cells were counterstained with DAPI (blue) to stain nuclei. Antibody dilution: 5 μg/mL.
Western Blotting Whole extracts of HEK-293T cells overexpressing Human MNK2 were separated on SDS-PAGE and probed with Anti-MNK2 (N-terminal) antibody produced in Rabbit (Cat. No. M0696). The antibody was developed using 1:30,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 4 μg/mL antibody 4. 2 μg/mL antibody + inhibition with immunizing peptide 5. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat (predicted), human, mouse (predicted) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 2-5 μg/mL using paraformadehyde fixed HEK-293T cells transfected with human MNK2 |
| immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells transfected with human MNK2 | |
| indirect immunofluorescence: suitable | |
| western blot: 1-2 μg/mL using lysates of HEK-293T cells transfected with human MNK2 | |
| UniProt accession no. | Q9HBH9  |
| Application: | Anti-MNK2 (N-terminal) antibody is suitable for use in immunocytochemistry (2-5 μg/mL using paraformaldehyde fixed, MNK2-transfected, HEK-293T cells) and indirect immunofluorescence. The antibody may also be used for immunoprecipitation (2-4 μg) and western blot (1-2 μg/mL) using lysates of HEK-293T cells transfected with human MNK2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | MNK2 is a serine threonine kinase that phosphorylates endogenous eIF4E and thereby regulates protein translation and cell growth. This kinase inhibits apoptotic responses induced by arsenic trioxide. MNK2 has two distinct splice forms, namely; MNK2a and MNK2b. The localization and function of MNK2 splice variants are directed by their N and C termini . Anti-MNK2 (N-terminal) antibody is specific for human MNK2. The antibody is also expected to react with mouse and rat MNK2. In immunoblotting, staining of the MNK2 band is specifically inhibited by the immunizing peptide. |
| Immunogen: | synthetic peptide corresponding to amino acids 51-64 of human MNK2 (corresponding to isoforms 1 and 2), conjugated to KLH via a C-terminal added cysteine residue. The corresponding sequence is conserved in human, rat, and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |