Anti-p38 MAP Kinase antibody produced in rabbit
SIGMA/M0800 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-ERK; Anti-ERK-2; Anti-ERK2; Anti-ERT1; Anti-MAPK2; Anti-NS13; Anti-P42MAPK; Anti-PRKM1; Anti-PRKM2; Anti-p38; Anti-p40; Anti-p41; Anti-p41mapk; Anti-p42-MAPK
MDL Number: MFCD00803556
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:10,000 Rabbit Anti-p38 MAP Kinase (Cat. No. M0800). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Rat Brain 2. NIH-3T3 3. JURKAT 4. HeLa 5. HEK-293
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 40 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:10,000 using NIH3T3 mouse fibroblast lysate and rat brain extract | |
| UniProt accession no. | P63086  |
| Application: | Anti-p38 MAP Kinase antibody has been used in western blotting (diluted 1:1000) using using NIH 3T3 mouse fibroblasts cell lysate and rat brain extract. It has also been used in microarray. |
| Biochem/physiol Actions: | Mitogen-activated protein kinases (MAP kinases, MAPKs) are a family of protein kinases which are considered to play a crucial role in signal transduction pathways in mammalian cells by leading mitogenic signals to their intracellular targets. MAP kinases regulate several cellular processes, such as proliferation, differentiation, response to stress and oncogenesis. p38 MAPK is activated in response to stress signals like- osmolarity changes, ultravoilet-irradiation, heat shock, cytokines and lipopolysaccharides (LPS). It is required for phosphorylation of MAPK-activated protein kinase 2 (MAPKAPK-2). Anti-p38 MAP Kinase antibody can be used to study the differential tissue expression, specific activation requirements and intracellular localization of MAP kinase isoforms in normal and neoplastic tissues. |
| Biochem/physiol Actions: | Rabbit anti-p38 MAP Kinase antibody reacts specifically with p38 MAP kinase from mouse and rat. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | P38 MAPK is a mitogen-activated protein kinase (MAPK) that belongs to serine/threonine protein kinases family and is activated in response to stress signals like- osmolarity changes, UV-irradiation, heat shock,cytokines and lipopolysaccharides (LPS). It mainly expresses in brain, muscle, thymus, spleen, heart, lung, kidney and fibroblasts. |
| Immunogen: | synthetic peptide K-DEVISFVPPPLDQEEMES derived from the C-terminus of mouse p38 MAP kinase (amino acids 343-360 with N-terminally added lysine) as immunogen. This sequence is highly conserved in human and rat p38 MAP kinase. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |