M-MLV Reverse Transcriptase
SIGMA/M1302 - Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis
Synonym: Moloney Murine Leukemia Virus Reverse Transcriptase
CAS Number: 9068-38-6
MDL Number: MFCD00283754
Product Type: Chemical
Representative packaging
| biological source | Porcine intestinal mucosa |
| color | colorless |
| concentration | 200 units/μL |
| feature | dNTPs included: no |
| hotstart: no | |
| form | liquid |
| input | purified RNA |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | RT-PCR: suitable |
| usage | sufficient for 200 reactions |
| Application: | M-MLV Reverse Transcriptase has been used: • for the preparation of cDNA libraries or for first strand cDNA synthesis • for use in a 2-step RT-PCR assay • in quantitative realtime-polymerase chain reaction (RT-qPCR) • in reverse transcription |
| Features and Benefits: | • Thermostable reverse transcriptase active at 37 °C. • Can be used to generate first strand cDNA of up to 7 kb. |
| General description: | Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand. |
| Legal Information: | Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. |
| Other Notes: | One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer. |
| Packaging: | Supplied with 10× M-MLV reverse transcriptase buffer containing DTT. |
| Preparation Note: | The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |