Monoclonal Anti-Maltose Binding Protein antibody produced in mouse
SIGMA/M1321 - clone MBP-17, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MBP
MDL Number: MFCD00282353
Product Type: Chemical
Immunoblotting Equal amounts of recombinant maltose-binding protein (MBP) were separated on SDS-PAGE and probed with serial concentrations of Monoclonal Anti-MBP (Cat. No. M1321). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg /mL antibody 2. 0.5 μg /mL antibody 3. 0.25 μg /mL antibody 4. 0.1 μg /mL antibody 5. Negative control – no primary antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MBP-17, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| indirect ELISA: suitable | |
| western blot: 0.05-0.1 μg/mL using purified recombinant MBP. |
| Application: | Monoclonal Anti-Maltose Binding Protein antibody produced in mouse has been used in: • immunoblotting • dot blot • luminometric immunoassay • enzyme linked immuno sorbent assay (ELISA) |
| Application: | Monoclonal Anti-Maltose Binding Protein antibody produced in mouse was used in agarose binding assay for the detection of activation-induced cytidine deaminase-MBP complex. |
| Biochem/physiol Actions: | Maltose binding protein (MBP) is a periplasmic binding protein that is important for nutrient uptake and chemotaxis of Escherichia coli. |
| Biochem/physiol Actions: | Maltose binding protein (MBP) tag creates a stable fusion product, that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. It facilitates the detection, isolation and purification of the proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the hybridoma MBP-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant MBP fusion protein. |
| General description: | The antibody recognizes native as well as denatured-reduced forms of purified MBP and MBP fusion proteins. |
| Immunogen: | Purified, recombinant MBP fusion protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |