Anti-Mus81 antibody, Mouse monoclonal
SIGMA/M1445 - clone MTA30 2G10/3, purified from hybridoma cell culture
Synonym: Anti-SLX3
MDL Number: MFCD08705592
Product Type: Chemical
Western Blotting Whole extract of HEK-293T cells were separated on SDS-PAGE and probed with Anti-Mus81 antibody, Mouse monoclonal, Clone: MTA30 2G10/3 (Cat. No. M1445). The antibody was developed using 1:3,000 Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304). Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. 1:2,000 antibody 4. 1:4,000 antibody 5. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MTA30 2G10/3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~59 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 0.5-1 μg/mL using HEK-293T total cell extract | |
| UniProt accession no. | Q96NY9  |
| Application: | Anti-Mus81 antibody, Mouse monoclonal has been used in immunofluorescence, immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Crossover junction endonuclease Mus81 is elevated in cells exposed to agents that block DNA replication. Knockout mice that are homozygous Mus81-/-, or their heterozygous cohorts Mus81-/+, are susceptible to spontaneous chromosomal damage and cancer predisposition suggesting that this protein is important for genomic integrity and tumor suppression |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Mus81 (mouse IgG2a isotype) is derived from the hybridoma MTA30 2G10/3 produced by the fusion of mouse myeloma cells (Sp2/0 Ag.14 cells) and splenocytes from BALB/c mice immunized with Mus81 protein. Crossover junction endonuclease Mus81 protein was identified by screening a human cerebellum cDNA library with a probe from the yeast Mus81. The human Mus81 has 551 amino acids with a molecular mass of 59 kDa. The highest sequence similarity between different species is around the VERKX3D motif. This motif is conserved in the endonuclease family (XPF) family of nucleases. The protein has helix-hairpin-helix DNA-binding domains in the N- and C-termini of the protein that are also conserved. |
| Immunogen: | Mus81 protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |