Malic Dehydrogenase from porcine heart
SIGMA/M1567 - ≥600 units/mg protein (biuret), ammonium sulfate suspension
Synonym: L-Malate: NAD+ oxidoreductase; MDH; Malate Dehydrogenase
EC Number: 232-622-5
MDL Number: MFCD00131567
Product Type: Chemical
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| foreign activity | Glutamic-Oxalacetic Transaminase ≤0.01% |
| Glutamic-Pyruvic Transaminase ≤0.01% | |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| specific activity | ≥600 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| Application: | Malic Dehydrogenase from porcine heart has been used: • in qualitative protein binding measurements • to test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pair • to investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase |
| Application: | Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid. |
| Biochem/physiol Actions: | Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH+. MDH present in the cytosol is involved in the shuttling of malate/aspartate. |
| General description: | Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity. |
| General description: | Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle. |
| General description: | Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells. |
| Other Notes: | One unit will convert 1.0 μmole of oxalacetate and β-NADH to |
| Physical form: | Suspension in 2.8 M (NH4)2SO4 solution, pH 6.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥600 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.37 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |