Anti-Monoamine Oxidase B antibody produced in rabbit
SIGMA/M1821 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Adrenalin oxidase; Anti-MAO, brain; Anti-MAO, platelet; Anti-Tyramine oxidase
Product Type: Chemical
Immunoblotting Tissue and cell lysates were separated on SDS-PAGE and blotted with Anti-MAO-B (Cat. No. M1821). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:1,000. Lanes 1,2. rat liver mitochondria 2. Inhibition with MAO (rat, 230-245) immunizing peptide (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~58 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1.0 μg/mL using rat liver mitochondrial fraction |
| UniProt accession no. | P27338  |
| Application: | Anti-Monoamine Oxidase B antibody has been used in western blotting. |
| Application: | Anti-Monoamine Oxidase B antibody produced in rabbit has also been used in coimmunoprecipitation (co-IP) and immunohistochemistry. |
| Biochem/physiol Actions: | Anti-Monoamine Oxidase B specifically recognizes human, mouse, and rat MAO-B. |
| Biochem/physiol Actions: | Monoamine oxidase B (MAO-B) is considered the predominant isoform responsible for dopamine metabolism in the human central nervous system (CNS). MAO-B activity correlates with personality traits. Alterations in MAO-B activity may underlie dopamine pathobiology in major depression. Selective MAO-B inhibitors elevate synaptosomal dopamine concentrations, and have recently shown clinical efficacy in the treatment of early Parkinson′s disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoamine Oxidase B (MAO-B) is a mitochondrial flavo enzyme. It is mainly found in the brain in dopaminergic, serotonergic, and histaminergic neurons and astrocytes. |
| General description: | Monoamine Oxidase B is encoded by the gene mapped to human chromosome Xp11.3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |