Monoclonal Anti-Macrophages antibody produced in mouse
SIGMA/M1919 - clone LN-5, ascites fluid
MDL Number: MFCD00282324
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:100 Monoclonal Anti-Macrophages antibody produced in Mouse, Clone: LN-5 (Cat. No. M1919) followed by 20 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LN-5, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using formalin-fixed, paraffin-embedded human tonsil sections |
| Biochem/physiol Actions: | Macrophages play a crucial role in body defense mechanisms and have phagocytic functionality. They are heterogeneous in morphology, cytochemistry, function and surface antigens. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and the diagnosis of various inflammation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human Macrophages (mouse IgM isotype) is derived from the LN-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Macrophages are widely distributed throughout the body. They belong to the mononuclear phagocytes family. Macrophages have abundant cytoplasm and are also termed histiocyte. |
| Immunogen: | deparaffinized human peripheral blood mononuclear cells |
| Physical form: | The product is provided as ascites fluid with 0.1% sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |