Anti-Monoamine Oxidase B (C-terminal) antibody produced in rabbit
SIGMA/M1946 - affinity isolated antibody, ~1.5 mg/mL, buffered aqueous solution
Synonym: Anti-Adrenalin oxidase; Anti-MAO, brain; Anti-MAO, platelet; Anti-Tyramine oxidase
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Monoamine Oxidase B (C-terminal) antibody produced in Rabbit (Cat. No. M1946) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Monoamine Oxidase B (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:4,000.
Immunoblotting Tissue and cell lysates were separated on SDS-PAGE and blotted with Anti-MAO-B (C-terminal) (Cat. No. M1946). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:4,000. Lanes 1,6. rat liver mitochondria 2,5. HepG2 3: HEK 293T cells transfected with human MAO-B 4. HEK 293T cells untransfected 5,6. Inhibition with MAO (human, 448-466) immunizing peptide (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~58 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.25-0.5 μg/mL using rat liver mitochondrial fraction and HEK-293T cells expressing human MAO-B |
| UniProt accession no. | P27338  |
| Application: | Anti-Monoamine Oxidase B (C-terminal) antibody produced in rabbit may be used in immunoblotting. |
| Biochem/physiol Actions: | Anti-Monoamine Oxidase B (C-terminal) specifically recognizes human, mouse and rat MAO-B. |
| Biochem/physiol Actions: | Monoamine oxidases (MAOs) are the main degradative enzymes responsible for the oxidative deamination of biogenic amines, such as epinephrine, norepinephrine, serotonin (5-HT) and dopamine in the central nervous system (CNS) and peripheral tissues. MAO-B is considered the predominant isoform responsible for dopamine metabolism in the human CNS. It participates in the metabolism of neuroactive and vasoactive amines. MAO-B activity correlates with personality traits. Alterations in MAO-B activity may underlie dopamine pathobiology in major depression. Selective MAO-B inhibitors elevate synaptosomal dopamine concentrations and have recently shown clinical efficacy in the treatment of early Parkinson′s disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Monoamine Oxidase B (C-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to human monoamine oxidase B (MAO-B) conjugated to KLH. Monoamine oxidases (MAOs) are mitochondrial flavoprotein enzymes, localized to the mitochondrial outer membrane. MAO-B is mainly found in the brain in dopaminergic, serotonergic and histaminergic neurons, as well as in astrocytes. It is a member of the flavin monoamine oxidase family. MAOB gene is mapped to human chromosome Xp11. |
| Physical form: | Supplied as a solution in 0.01 M PBS, pH 7.4, containing 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |