4-Methylumbelliferyl N-acetyl-β-D-glucosaminide

SIGMA/M2133 - β-N-acetylhexosaminidase substrate

Synonym: 4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside

CAS Number: 37067-30-4
Empirical Formula (Hill Notation): C₁₈H₂₁NO₈
Molecular Weight: 379.36
EC Number: 253-333-0
Linear Formula: C₁₈H₂₁NO₈
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-M2133-25MG	25 mg	$71.50 1/EA	Add To Favorites
45-M2133-100MG	100 mg	$210.00 1/EA	Add To Favorites
45-M2133-250MG	250 mg	$348.00 1/EA	Add To Favorites
45-M2133-1G	1 g	$1110.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M2133-100MG

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (TLC)
form	powder
InChI	1S/C18H21NO8/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21)/t13-,15-,16-,17-,18?/m1/s1
InChI key	QCTHLCFVVACBSA-XWEABGILSA-N
Quality Level	200
SMILES string	CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc3C(C)=CC(=O)Oc3c2
solubility	DMF: 20 mg/mL, clear, colorless to faintly yellow
storage temp.	−20°C

Application:	4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a substrate for assaying • β-N-acetylglucosaminidase (NAGase) from soil samples • chitinase from Fusarium oxysporum using fluorimetric assay • β-N-acetylhexosaminidases from Arabidopsis thaliana roots and leaves samples
Biochem/physiol Actions:	4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a synthetic fluorogenic substrate for N-acetyl-β-D-glucosaminidase (NAGase). The enzyme functionality is assessed by its ability to hydrolyze the N-acetyl-D-glucosamine (NAG) residue in 4-MUF-NAG. 4-MUF-NAG is also a substrate for Chitinase and N-acetylhexosaminidase.
General description:	4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) contains chitin monomer, N-acetyl-D-glucosamine (NAG) and a 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF) moiety.
General description:	Fluorogenic substrate for β-N-acetylhexosaminidase
Packaging:	1 g in poly bottle
Packaging:	25, 100, 250 mg in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (TLC)
Storage Temp.	−20°C
UNSPSC	12352204

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