Medium 199
SIGMA/M2154 - With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym: M199 Medium; TCM 199
Product Type: Chemical
| components | sodium pyruvate: no L-glutamine: no NaHCO3: yes HEPES: no phenol red: yes Earle’s salts (5% CO2): yes |
| form | liquid |
| impurities | endotoxin, tested |
| pH | 7.0-7.6 (25 °C, 209 g/L) |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Medium 199 has been used:• to culture rumen epithelial cells from yearling Speckle Park beef heifers • as a component of the maturation medium for the incubation of Cumulus oocyte complexes (COCs) from bovine ovaries • as a component of the Dulbecco′s modified Eagle medium (DMEM) mixture to maintain rat neonatal cardiac myocytes (NCM) |
| General description: | Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues. |
| Preparation Note: | Supplement with 0.1 g/L |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |