Minimum Essential Medium Eagle
SIGMA/M2279 - With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym: EMEM; MEM
Product Type: Chemical
| components | phenol red: yes sodium pyruvate: no L-glutamine: no HEPES: no NaHCO3: yes Earle’s salts (5% CO2): yes |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Minimum Essential Medium Eagle has been used to culture the:• explants of aortic valve leaflets • human liver cancer cell line (HepG2) cells • human fetal brain progenitor-derived astrocyte cells (PDA) • breast cancer cell line (MCF-7) • human glioblastoma cell line (T98G) |
| General description: | Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. It includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for the cultivation of a wide variety of cells grown in monolayers and attached cell lines, such as fibroblasts, in the presence of fetal bovine serum (FBS), calf, or horse sera. |
| Preparation Note: | Supplement with 0.292 g/L L-glutamine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |