Anti-MAP2 (2a+2b) antibody, Mouse monoclonal
SIGMA/M2320 - ~2 mg/mL, clone AP-20, purified from hybridoma cell culture
Synonym: Anti-MAP2 (2a+2b) antibody, Mouse monoclonal; Anti-Microtubule Associated Protein 2
MDL Number: MFCD00164684
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Efficient reprogramming of mouse fibroblasts to neuronal cells including dopaminergic neurons. By: Oh, S. I., Park, H. S., et al. in ScientificWorldJournal, 2014. PubMed ID: 24991651 Image collected and cropped by CiteAb from the following publication, (http://www.hindawi.com/journals/tswj/2014/957548/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: TLX activates MMP-2, promotes self-renewal of tumor spheres in neuroblastoma and correlates with poor patient survival. By: Chavali, P. L., Saini, R. K., et al. in Cell Death Dis, 2014. PubMed ID: 25356871 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/cddis2014449), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Stainig of formalin-fixed, paraffin-embedded human brain section with 20 μg/mL Monoclonal Anti-MAP2 (2a+2b), Clone: AP-20 (Cat. No. M2320) using biotinExtrAvidin®-Peroxidase.
Immunohistochemistry Stainig of formalin-fixed, paraffin-embedded mouse brain section with 20 μg/mL Monoclonal Anti-MAP2 (2a+2b), Clone: AP-20 (Cat. No. M2320) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Adult and Newborn Rat brain extract was separated on SDS-PAGE and probed with Monoclonal PA to MAP2 (MO) CL: AP-20 (Cat. No. M2320). The antibody was developed with HRP APA Mouse Fab ads/HIgG (gt) (Cat. No. A9917) and an ECL substrate. Antibody dilution 3 μg/mL
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AP-20, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | apparent mol wt 280 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | Xenopus, mouse, quail, human, bovine, rat, aquatic salamander |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| microarray: suitable | |
| western blot: 1-3 μg/mL using rat brain preparation or rat cerebral cortex extract | |
| UniProt accession no. | P11137  |
| Application: | Monoclonal Anti-MAP2 (2a+2b) antibody produced in mouse has been used in immunocytochemistry. |
| Application: | Mouse monoclonal clone AP-20 anti-MAP2 (2a+2b) antibody is used to tag MAP2 for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of MAP2a and MAP2b in cell signaling especially in neural tissues. |
| Biochem/physiol Actions: | Microtubule associated protein 2 (MAP2) is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton. MAP2 is degraded by a cathepsin D like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in others. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20 MAP2a appears, at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in others. |
| General description: | Monoclonal Anti-MAP2 (2a + 2b) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with bovine microtubule associated protein 2 (MAP2). |
| General description: | Monoclonal Anti-MAP2 specificity was established by an immunoblot. The antibody localizes the high molecular weight forms of MAP2, namely MAP2a and MAP2b, but shows no reactivity with MAP2c. No crossreactivity is observed with MAP1, MAP5, tubulin, or tau (t). |
| Immunogen: | bovine MAP2 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Monoclonal Anti-MAP2 antibody reacts with human, bovine, rat, mouse, Xenopus, salamander, and quail tissue or cells by immunocytochemical techniques utilizing either a fluorescent or peroxidase label, or by immunohistochemistry. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |