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4-Methylumbelliferyl β-D-lactopyranoside

SIGMA/M2405 - β-galactosidase substrate

Synonym: 4-Methylumbelliferyl β-D-lactoside

CAS Number: 84325-23-5
Empirical Formula (Hill Notation): C22H28O13
Molecular Weight: 500.45
MDL Number: MFCD00083406
Linear Formula: C22H28O13
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-M2405-25MG 25 mg
 $762.00
1/EA		 Add To Favorites
45-M2405-100MG 100 mg
 $2340.00
1/EA		 Add To Favorites
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M2405-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M2405-25MG

 

assay ≥98.00% (TLC)
fluorescence λex 365 nm; λem 445 nm (after enzymatic degradation)
form powder
InChI 1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15+,16+,17-,18-,19-,20-,21-,22+/m1/s1
InChI key PRTGXBPFDYMIJH-KSFLKEQHSA-N
Quality Level 200 
SMILES string CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12
solubility water: 9.80-10.20 mg/mL, clear, colorless to faintly yellow
storage temp. −20°C
Application: 4-Methylumbelliferyl β-D-lactopyranoside has been used as a chromophoric substrate for cellobiohydrolase I (CBHI) in T. reesei. It has also been used as a substrate for cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B) and β-glucosidase from fermented samples in enzymatic assay.
Biochem/physiol Actions: 4-Methylumbelliferyl β-D-lactopyranoside is a fluorescence compound hydrolyzed by the enzyme β-glucosidase (βG) and CBH1
Packaging: 25, 100 mg in glass bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥98.00% (TLC)
Storage Temp. −20°C
UNSPSC 12352204

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