Anti-MDC1 antibody, Mouse monoclonal
SIGMA/M2444 - clone MDC1-50, purified from hybridoma cell culture
Synonym: Anti-Mediator of DNA Damage Checkpoint Protein 1
MDL Number: MFCD07370941
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2.5 μg/mL Monoclonal Anti-MDC1, Clone: MDC1-50 (Cat. No. M2444). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunoblotting Whole extract of G361 cell was separated on SDS-PAGE and probed with Monoclonal Anti-MDC1 Clone: MDC1-50 (Cat. No. M2444). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. Negative control: without 1st antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MDC1-50, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~250 kDa (2-3 bands) |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using total cell extract of G361 cells | |
| UniProt accession no. | Q14676  |
| Application: | Monoclonal Anti-MDC1 antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA • immunofluorescence • western blotting • immunoprecipitation • immunocytochemistry |
| Application: | Mouse monoclonal clone MDC1-50 anti-MDC1 antibody is used to tag mediator of DNA damage checkpoint protein 1 for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques such as ELISA, immunoblotting (approx. 250 kDa, 2-3 bands), immunoprecipitation and immunocytochemistry. It is used as a probe to determine the roles of mediator of DNA damage checkpoint protein 1 in DNA repair and genomic stability. |
| Biochem/physiol Actions: | Genomic instability caused by the disruption of mechanisms that regulate cell-cycle checkpoints, DNA repair and apoptosis may lead to the development of cancer. ATM and ATR protein kinases are mediated to DNA damage sites by molecular adapters or mediators proteins such as Histone H2AX, Claspin and BRCTmotif containing molecules such as 53BP1, BRCA1, and MDC1 (mediator of DNA damage checkpoint protein 1). MDC1 interacts with the MRE11 complex (containing MRE11, RAD50 and NBS1 proteins). The MRE11 complex is involved in the detection repair and signaling of DNA damage. Upon ionizing radiation MDC1 is hyperphosphorylated by ATM and localizes to nuclear foci together with the MRE11 complex, phosphorylated H2AX and 53BP1. A radio resistant DNA synthesis (RDS) phenotype in cells is formed by down regulation of MDC1 protein expression by siRNA. |
| Biochem/physiol Actions: | Mouse monoclonal clone MDC1-50 anti-MDC1 antibody recognizes human and monkey MDC1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-MDC1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a recombinant human MDC1 fragment. Mediator of DNA damage checkpoint protein 1 (MDC1) contains 2089 amino-acid residues with a predicted molecular weight of 226.4 kDa. The protein contains an FHA (forkhead-associated) domain at its amino terminus and two BRCT (BRCA1 carboxyl terminal) domains at its carboxy terminus. |
| Immunogen: | recombinant human MDC1 fragement (amino acids 2-200). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |