Medium 199
SIGMA/M2520 - HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym: M199 Medium; TCM 199
Product Type: Chemical
| components | L-glutamine: yes phenol red: yes Earle’s salts (5% CO2): yes NaHCO3: no sodium pyruvate: no HEPES: 25 mM |
| form | powder |
| Quality Level | 500  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Medium 199 has been used to: • maintain the cumulus-oocyte complex (COC) before maturation and for culturing of these oocytes for in vitro maturation • to rinse the aspiration line during the collection of the ovum from German Holstein cows • to culture cardiac endothelial cells from rats |
| General description: | Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues. |
| Preparation Note: | Formulated to contain 14.7 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 2.2 g/L sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |