Malic Dehydrogenase from porcine heart
SIGMA/M2634 - buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)
Synonym: L-Malate: NAD+ oxidoreductase; MDH; Malate Dehydrogenase
MDL Number: MFCD00131567
Product Type: Chemical
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| cation traces | NH4+: ≤10 μg/mg protein |
| foreign activity | Glutamic-Oxalacetic Transaminase ≤0.01% |
| Glutamic-Pyruvic Transaminase ≤0.01% | |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | 600-1000 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| Application: | Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens. |
| Biochem/physiol Actions: | Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form. The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD |
| Biochem/physiol Actions: | Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C. |
| General description: | Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle. |
| General description: | Mitochondrial Isozyme |
| Other Notes: | One unit will convert 1.0 μmole of oxalacetate and β-NADH to |
| Physical form: | Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 600-1000 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.37 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |