Anti-Munc-18-1 antibody produced in rabbit
SIGMA/M2694 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-Munc-18α; Anti-STXBP1; Anti-
MDL Number: MFCD07370408
Product Type: Chemical
Immunofluorescence NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:250 Anti-Munc-18-1 antibody produced in Rabbit (Cat. No. M2694) followed by 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887). Picture B: cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-LIM Kinase 1 antibody produced in Rabbit (Cat. No. L2290). The antibody was developed using 1:10,000 Peroxidase Goat Anti-Rabbit IgG. Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. 1:4,000 antibody 4. 1:2,000 antibody + inhibition with immunizing peptide 5. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 67 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:250-1:500 using mouse fibroblast NIH3T3 cell line |
| microarray: suitable | |
| western blot: 1:2,000-1:4,000 using rat brain extract (S1 fraction) and PC12 whole cell extract. | |
| UniProt accession no. | O08599  |
| Application: | Anti-Munc-18-1 antibody produced in rabbit has been used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Munc-18-1 functions in synaptic vesicle exocytosis. It modulates neurotransmission by associating with syntaxin 1. Munc-18-1 binds to both syntaxin 1A and 1B isoforms, inhibiting the binding of t-soluble N-ethylmaleimide-sensitive factor-attached protein receptor (SNARE) synaptobrevin/vesicle-ass |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Munc-18-1 (67 kDa) is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin. It is primarily expressed in neuronal tissues. Both Munc-18-1 and 2 isoforms bind tightly to syntaxins 1A, 2, and 3. |
| Immunogen: | synthetic peptide corresponding to amino acids 577-594 located at the C-terminus of rat Munc-18-1 conjugated to KLH. This sequence is identical in mouse, chicken, bovine, and human Munc-18-1. It does not share homology with the Munc-18-2 and Munc-18-3 isoforms. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |