Murashige and Skoog Modified Basal Salt Mixture
SIGMA/M2909 - powder, suitable for plant cell culture
Synonym: Murashige and Skoog Modified Basal Salt Mixture, w/o Ammonium Nitrate
Product Type: Chemical
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| application(s) | agriculture |
| form | powder |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | cell culture | plant: suitable |
| Application: | Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter). |
| Application: | Murashige and Skoog Modified Basal Salt Mixture has been used: • to promote root development for internode stem suckers • to promote shoot growth for the calluses • for low nitrogen (LN) treatment, for the growth of Arabidopsis thaliana |
| Preparation Note: | Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M2909) contain the macronutrients and micronutrients of the original classic formulation without ammonium nitrate. It can be combined with M&S vitamins or Gamborg’s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture. |
| Preparation Note: | Without NH4NO3 With the macro- and micronutrients described by Murashige and Skoog (1962). Media Formulation |
| Symbol |  GHS03 |
| Signal word | Warning |
| Hazard statements | H272 |
| Precautionary statements | P210 - P220 - P280 - P370 + P378 - P501 |
| Hazard Codes | O |
| Risk Statements | 8 |
| RIDADR | UN 1477 5.1 / PGII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |