Myokinase from rabbit muscle
SIGMA/M3003 - ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)
Synonym: ATP: AMP phosphotransferase; Adenylate Kinase
CAS Number: 9013-02-9
EC Number: 232-743-3
MDL Number: MFCD00131644
Product Type: Chemical
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| biological source | rabbit muscle |
| foreign activity | 3-phosphoglyceric phosphokinase ≤0.05% |
| lactic dehydrogenase and pyruvate kinase ≤0.01% | |
| form | ammonium sulfate suspension |
| mol wt | 21 kDa |
| Quality Level | 200  |
| specific activity | 1,500-3,000 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| Application: | Myokinase has been used: • in a study to assess its reaction with the mercurials p-hydroxymercuribenzoate and p-mercuribenzenesulfonate • in a study to investigate the ligand-binding properties of its peptide fragments • as one of the components in the ATP generation solution for the quantification of 3’3’-c-di-AMP using an AMP-Luciferase assay |
| Biochem/physiol Actions: | Myokinase or adenylate kinases, play a crucial role in regulating energy metabolism within cells and balancing adenine nucleotide ratios in different cellular compartments. Myokinase is a key enzyme involved with adenine nucleotide metabolism. The action of myokinase insures that the adenine nucleotides (ATP, ADP, and AMP) are maintained in equilibrium in the cytosol. Myokinase catalyzes the following reaction:ADP + ADP → ATP + AMP |
| Biochem/physiol Actions: | Rabbit muscle myokinase can be inactivated by treatment with dithiobis-(2-nitrobenzoic |
| General description: | Research area: Cell Signaling Myokinase, also called Adenylate kinase or ADK, is a phosphotransferase enzyme. It belongs to the family of nucleoside monophosphate kinases. Myokinase is an enzyme that can be found in skeletal muscle and acts as a phosphotransferase agitator. |
| Other Notes: | One unit will convert 2.0 μmoles of ADP to ATP+AMP per min at pH 7.6 at 37°C. |
| Physical form: | Suspension in 3.2 M (NH4)2SO4 solution containing 0.001 M EDTA, pH 6.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 1,500-3,000 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.7.4.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |