Minimum Essential Medium Eagle
SIGMA/M3024 - with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture
Synonym: EMEM; MEM
Product Type: Chemical
| components | Earle’s salts (5% CO2): yes L-glutamine: no HEPES: no NaHCO3: no sodium pyruvate: no phenol red: no |
| form | powder |
| Quality Level | 500  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Minimum Essential Medium Eagle has been used: • to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies • to culture MCF-7 cells for experimental treatments with hormone • to maintain zebrafish pineal glands for action spectrum analysis |
| Features and Benefits: | Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells |
| General description: | Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers. |
| Preparation Note: | Formulated to contain 9.3 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 0.292 g/L |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |