MES sodium salt

SIGMA/M3058 - BioPerformance Certified, suitable for cell culture

Synonym: 4-Morpholineethanesulfonic acid, sodium salt; Sodium 2-Morpholinoethanesulfonate; 2-(N-Morpholino)ethanesulfonic acid sodium salt; 4-Morpholineethanesulfonic acid sodium salt

CAS Number: 71119-23-8
Empirical Formula (Hill Notation): C₆H₁₂NNaO₄S
Molecular Weight: 217.22
EC Number: 275-203-2
MDL Number: MFCD00065473
Linear Formula: C₆H₁₂NNaO₄S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-M3058-25G	25 g	$79.30 1/EA	Add To Favorites
45-M3058-100G	100 g	$255.00 1/EA	Add To Favorites
45-M3058-500G	500 g	$984.00 1/EA	Add To Favorites
45-M3058-1KG	1 kg	$1720.00 1/EA	Add To Favorites
45-M3058-10KG	10 kg	$8380.00 1/EA	Add To Favorites

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This image depicts SKU: M3058-25G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	agriculture diagnostic assay manufacturing life science and biopharma sample preparation
color	white
foreign activity	DNase, NICKase, RNase, protease, none detected
form	crystalline powder
grade	BioPerformance Certified
	Molecular Biology
impurities	endotoxin and total aerobic microbial count, tested
InChI	1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1
InChI key	IRHWMYKYLWNHTL-UHFFFAOYSA-M
pKa	6.1
Quality Level	400
SMILES string	[Na+].[O-]S(=O)(=O)CCN1CCOCC1
solubility	water: 335.3 g/L at 20 °C
storage condition	dry at room temperature
suitability	suitable for component for culture media
	suitable for molecular biology
technique(s)	cell culture \| mammalian: suitable
useful pH range	5.5-6.7

Application:	MES sodium salt has been employed as a coupling buffer for amine-reactive sensors at either pH 4.5 or pH 5.0 to facilitate the coupling of monoclonal antibodies (MAbs) onto the sensors, used in hybridization studies with fluorescently-labeled complementary strands and microarray slide washing during microarray analysis, and played a role in preparing a new set of RNA phosphoramidites compatible with in situ photolithography for growing RNA oligonucleotides on microarrays.
Application:	MES sodium salt has been used as a coupling buffer during the coupling of MAbs onto amine-reactive sensors. It has also been used as a component of germination medium and shoot induction medium during in vitro plant regeneration.
Features and Benefits:	• Suitable for Molecular Biology and Cell Culture • Tested for Endotoxins and Total Aerobic Microbial Count • Free from DNase, NICKase, RNase, and Protease • Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
General description:	4-Morpholineethanesulfonic acid, sodium salt (MES) is a popular zwitterionic buffer in scientific research due to its excellent properties. It can buffer solutions effectively within a pH range of 5.5-6.5 with a pKa value of 6.1. This product is BioPerformance certified and it finds extensive application as a buffering agent in biochemistry and molecular biology experiments, contributing to the stabilization of pH in different biological reactions including western blotting, cell culture and electrophoresis.
General description:	MES is one of the so-called "Good" buffers developed for biological applications. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1 kg in poly bottle
Packaging:	25, 100, 500 g in poly bottle
Preparation Note:	MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions are stable at 2-8°C for months. Sterilization should be done by filteration through 0.2μm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

UNSPSC

12161700

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