Monoclonal Anti-MBNL1 antibody produced in mouse
SIGMA/M3320 - ~1 mg/mL, clone HL 1822 (3A4-1E9), purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CUG Triplet Repeat Expansion Double-stranded RNA-binding protein; Anti-EXP; Anti-KIAA0428; Anti-
MDL Number: MFCD09039233
Product Type: Chemical
Immunoblotting HeLa nuclear cells extract was separated on SDS-PAGE, blotted with Monoclonal Anti-MBNL1 Clone: HL1822 (3A4-1E9) (Cat. No. M3320) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.2 μg/mL antibody 2. 0.5 μg/mL antibody
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:1,000 Monoclonal AntI-MBNL1, Clone: HL 1822 (3A4-1E9) (Cat. No. M3320). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. L929 2. COS7 3. CHO 4. MDCK
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HL 1822 (3A4-1E9), monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified, unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| microarray: suitable | |
| western blot: 0.2-0.5 μg/mL using HeLa nuclear cell extract | |
| UniProt accession no. | Q9NR56  |
| Application: | Monoclonal Anti-MBNL1 antibody produced in mouse has been used in: immunoblotting, immunohistochemistry and immunocytochemistry |
| Biochem/physiol Actions: | Proteins of the MBNL (muscleblind-like) family enhance inclusion or exclusion of specific exons on different pre-mRNAs by inhibiting the activity of CUG-BP and ETR-3-like factors (CELF proteins) bound to distinct intronic sites. MBNL1 may be implicated in the stimulation of muscle differentiation. Mice knockouts for the MBNL1 gene develop muscle, eye, and RNA splicing abnormalities that are characteristic of the neuromuscular disease myotonic dystrophy (DM) disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Muscle blind-like 1 (MBNL1) is a homolog of human muscle blind like proteins. Proteins of the MBNL (muscleblind-like) family are mainly expressed in skeletal muscle neuronal tissues, thymus, liver and kidney. |
| Immunogen: | recombinant muscleblind-like1 (MBNL1) fusion protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |