Anti-Myosin Iβ (Nuclear) antibody produced in rabbit
SIGMA/M3567 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Myh 2; Anti-Myo1c; Anti-Myr 2
MDL Number: MFCD05665241
Product Type: Chemical
Immunofluorescence NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:50 Anti-Myosin I-BETA (Nuclear) antibody produced in Rabbit (Cat. No. M3567) followed by 1:500 Anti-Rabbit IgG-Cy3. (B) cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of Dog MDCK cells were separated on SDS-PAGE and probed with Anti-Myosin I-β (Nuclear) antibody produced in Rabbit (Cat. No. M3567). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:2,000 antibody 2. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~120 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, canine, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:50 using mouse NIH3T3 cells |
| microarray: suitable | |
| western blot: 1:1,000 using extract of human HeLa cells nuclei and dog MDCK cells | |
| UniProt accession no. | O00159  |
| Application: | Anti-Myosin Iβ (Nuclear) antibody produced in rabbit has been used in: • western blot • confocal immunofluorescence • immunogold labeling • immunofluorescence • co-immunoprecipitation experiments • immunostaining |
| Biochem/physiol Actions: | Myosin I is involved in cell motility, microvilli anchorage, vesicular and organelle transport and signal transduction. Myosin I associate with lipid membranes through its tail domain while its motor domain interacts with actin filaments. Myosin Iβ (nuclear) co-localizes with RNA polymerases I and II and possibly acts as a molecular motor to power transcription. |
| Biochem/physiol Actions: | Recognizes the heavy chain of the nuclear form of human myosin Iβ. Additional bands may be detected in some preparations. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myosins belong to a superfamily of actin-based motor proteins comprising to date at least 15 classes. Myosin I (also designated myosin-1c, myo1c, M1C, myr 2 and myh 2) is a widely distributed single-headed myosin composed of an N-terminal motor domain, a calmodulin-light chain binding neck region, and a short C-terminal domain. In mammalian cells, myosin I is usually found in the cytoplasm and is especially enriched in perinuclear regions and dynamic cell margins. Myosin Iβ is found in a variety of cells such as kidney tubular cells and inner-ear hair cells. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 1-16 of the heavy chain of mouse myosin Iβ (nuclear form). This sequence is missing from the other myosin I isoforms as well as from other currently known myosins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |