Anti-MAP Kinase, Monophosphorylated Tyrosine antibody ,Mouse monoclonal
SIGMA/M3682 - clone ERK-PY193, purified from hybridoma cell culture
Synonym: Anti-pY-ERK
MDL Number: MFCD02096848
Product Type: Chemical
Immunoprecipitation Monoclonal Anti-MAP Kinase, Monophosphorylated Tyrosine, Clone: ERK-PY193 (Cat. No. M3682) was used to immunoprecipitate MAPK1/MAPK3 (pY-ERK) from HeLa whole cell lysate. Detection antibody: Rabbit Anti-MAP Kinase (ERK-1, ERK-2) (Cat. No. M5670) followed by Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 20 μg antibody 2. Negative control: 20 μg nonrelevant antibody 3. Positive control: HeLa whole cell lysate
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ERK-PY193, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen, ERK-1 44 kDa |
| antigen, ERK-2 42 kDa | |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr) |
| technique(s) | capture ELISA: suitable |
| immunocytochemistry: suitable | |
| microarray: suitable | |
| western blot: 1-5 μg/mL using cell extract of rat fibroblasts cell line, Rat1, activated with sorbitol | |
| UniProt accession no. | P28482   |
| Application: | Monoclonal Anti-MAP Kinase, Monophosphorylated Tyrosine antibody has been used in: • immunoblotting • enzyme linked immunosorbent assay (ELISA) • dot-blot • immunocytochemistry • western blotting • immunofluorescence |
| Biochem/physiol Actions: | MAPK kinase (MAPKK) is the immediate upstream activator of the MAPK, MAPKK kinase (MAP3K), and MAP3K kinase (MAP4K) for the enzymes further upstream, respectively. The kinases in the MAPK level are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. |
| Biochem/physiol Actions: | The antibody reacts with the monophosphorylated tyrosine form of MAP kinases (ERK1 and ERK2). It does not recognize the non-phosphorylated, diphosphorylated, and the monophosphorylated threonine forms of ERK/MAP kinases, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated tyrosine residue within the regulatory site of MAP kinase (e.g.,Tyr185 in ERK-2). Cross-reactivity has been observed with the monophosphorylated tyrosine peptide of JNK. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-MAP Kinase, Mono-phosphorylated Tyrosine (pY-ERK) (mouse IgG1 isotype) is derived from the ERK-PY193 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide sequence corresponding to the phosphorylated form of ERK-activation loop, conjugated to KLH. The mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in many signaling pathways. This family includes the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK (c-Jun N-terminal protein kinase, also termed stress-activated protein kinase, SAPK1), and p38 MAPK (also termed SAPK2) subfamilies. |
| Immunogen: | synthetic peptide HTGFLTEpYVAT, corresponding to the phosphorylated form of ERK-activation loop. |
| Physical form: | Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Prepared from a culture supernatant of bioreactor grown hybridoma. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |