SigmaMarker™
SIGMA/M3913 - low range, mol wt 6,500-66,000 Da
Synonym: protein markers; protein standards
MDL Number: MFCD00165844
Product Type: Chemical
| form | lyophilized powder |
| mol wt | 6,500-66,000 Da |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| usage | sufficient for 10-20 large gels (16 × 18 cm) |
| sufficient for 20-30 mini-gels (10 × 10 cm) | |
| 1 vial sufficient for 50-100 PhastGels |
| Application: | SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining. |
| Legal Information: | SigmaMarker is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Protein (mol. wt.) Aprotinin, bovine lung (6,500) α-Lactalbumin, bovine milk (14,200) Trypsin inhibitor, soybean (20,000) Trypsinogen, bovine pancreas (24,000) Carbonic anhydrase, bovine erythrocytes (29,000) Glyceraldehyde-3-phosphat Ovalbumin, chicken egg (45,000) Albumin, bovine serum (66,000) |
| Other Notes: | Will yield eight protein bands with Coomassie Blue staining. |
| Preparation Note: | Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue. |
| Symbol |   GHS05,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H317 - H318 - H334 - H412 |
| Precautionary statements | P261 - P273 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | F,Xn |
| Risk Statements | 11-20/22-37/38-41 |
| Safety Statements | 26-39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41105337 |