MegaCell™ Dulbecco’s Modified Eagle’s Medium
SIGMA/M3942 - liquid, sterile-filtered, suitable for cell culture
Synonym: DMEM; DMEM Supplement
Product Type: Chemical
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| components | L-glutamine: no glucose: 4500 mg/L (Dextro) sodium pyruvate: yes |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 300  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | MegaCell™ Dulbecco′s Modified Eagle′s Medium has been used: • as a component of the M10 medium to culture CD133+ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients • as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells • to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies |
| General description: | MegaCell™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines. |
| Legal Information: | MegaCell is a trademark of Promega Corp. |
| Legal Information: | Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation. |
| Preparation Note: | Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |