Anti-Mouse IgG (Fab specific) antibody produced in goat
SIGMA/M4155 - 2.0 mg/mL, affinity isolated antibody
Synonym: Anti-Mouse Fab Antibody; Anti-Mouse Fab Antibody - Anti-Mouse IgG (Fab specific) antibody produced in goat
MDL Number: MFCD00162619
Product Type: Chemical
ELISA Isotype specifity test: 0.3 μg/mL Goat Anti-Mouse IgG (Fab specific) (Cat. No. M4155) was used to detect the Fab fragment of Kappa (Cat. No. M9035) and Lambda (Cat. No. M6034) IgGs from murine myeloma. The antibody was developed using Anti-Goat IgG (whole molecule)-Peroxidase (Cat. No. A4174) and a chromogenic substrate. Columns 1. Antibody dilution: 1:3,750 2. Antibody dilution: 1:7,500 3. Antibody dilution: 1:15,000 4. Negative control: normal goat serum: 1:3,750
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 2.0 mg/mL |
| conjugate | unconjugated |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:15,000 |
| Ouchterlony double diffusion: suitable |
| Application: | Anti-Mouse IgG (Fab specific) antibody produced in goat has been used in the expression and purification of human platelet factor 4 (PF4) and anti-PF4 monoclonal antibodies. |
| Application: | Anti-Mouse IgG (Fab specific) antibody produced in goat was used as secondary antibody in immunocytochemical staining at a dilution of 1:10. |
| Biochem/physiol Actions: | IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a critical role in antibody-dependent cell-mediated cytotoxicity (ADCC). It has the longest serum half life and might be associated with allergy. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. It provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds. IgG has four isotypes IgG1, IgG2, IgG3 and IgG4. |
| Other Notes: | Antibody adsorbed with bovine, equine and human serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |