Methylene Blue hydrate
SIGMA/M4159 - suitable for nucleic acid staining, BioReagent
Synonym: 3,7-
CAS Number: 122965-43-9
Empirical Formula (Hill Notation): C16H18ClN3S · xH2O
Molecular Weight: 319.85 (anhydrous basis)
EC Number: 200-515-2
MDL Number: MFCD00150006
Linear Formula: C16H18ClN3S · xH2O
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M4159-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M4159-25G
| form | powder |
| grade | Molecular Biology |
| InChI | 1S/C16H18N3S.ClH.H2O/c1-1 |
| InChI key | WQVSELLRAGBDLX-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 100  |
| SMILES string | O.[Cl-].CN(C)c1ccc2N=C3C= |
| solubility | water: 1 mg/mL (dark blue) |
| storage temp. | room temp |
| suitability | suitable for nucleic acid staining |
| technique(s) | nucleic acid detection: suitable |
| Application: | Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells. |
| Features and Benefits: | • Less hazardous than staining with ethidium bromide • Viewable in visible light • Fast and easy to use |
| General description: | Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories. |
| Packaging: | 25, 100 g in glass bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |