Monoclonal Anti-MAP1 antibody produced in mouse
SIGMA/M4278 - clone HM-1, ascites fluid
Synonym: Anti-MAP1a
MDL Number: MFCD00164681
Product Type: Chemical
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Monoclonal Anti-MAP1 antibody produced in Mouse, Clone: HM-1 (Cat. No. M4278). The antibody was developed using 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HM-1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): suitable |
| microarray: suitable | |
| western blot: 1:500 using a fresh total rat brain extract or an enriched microtubule protein preparation | |
| UniProt accession no. | Q9QYR6  |
| Application: | Monoclonal Anti-MAP1 has been used in : • immunoblotting • dot blot • immunocytochemistry • immunohistochemistry |
| Biochem/physiol Actions: | Microtubules are the ubiquitous cytoskeletal structural components that are involved in intracellular transport. They are composed of tubulin and microtubule-associated proteins (MAPs). MAPs are known to mediate the binding of membranous organelles, actin filaments and intermediate filaments to microtubules. Therefore, it might be important for cellular processes such as mitosis and organelle transport, and for determining the dynamic properties of the cytoskeleton. |
| Biochem/physiol Actions: | The antibody does not cross-react with other MAPs or tubulin. By immunohistochemical staining of brain tissue, the antibody shows selective labeling of neurons with stronger staining of axons than dendrites. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-MAP1 (mouse IgG1 isotype) is derived from the HM-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a rat brain MAPs preparation. MAP1 is one of the major neuronal MAPs as well as being the largest (350 kD). MAP1 is more generally distributed, being found in both dendrites and axons of neurons and in glial cells in brain, in chromatophores and on both interphase and mitotic microtubules in various tissue culture cells. |
| Immunogen: | rat brain microtubule-associated proteins (MAPs) |
| WGK Germany | 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |