Anti-Mouse IgG (Fc specific) antibody produced in goat
SIGMA/M4280 - 2.0 mg/mL, affinity isolated antibody
Synonym: Anti Mouse Fc Antibody; Anti Mouse Fc Antibody - Anti-Mouse IgG (Fc specific) antibody produced in goat
MDL Number: MFCD00162625
Product Type: Chemical
ELISA 10 μg/mL Anti-Mouse IgG (Fc specific) antibody produced in Goat (Cat. No. M4280), followed by 1:5,000 Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A4187), was probed to detect Mouse IgG by indirect ELISA. Reactivity is observed with Mouse IgG and Mouse FC but not with Mouse Fab, Human IgG, Human IgA, Human IgM, Bovine IgG, Bovine IgM and Horse IgG.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 2.0 mg/mL |
| conjugate | unconjugated |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:20,000 |
| Application: | Anti-Mouse IgG (Fc specific) antibody produced in goat has been used in: • enzyme-linked immunosorbent assay (ELISA) • flow cytometry • immunoprecipitation • immunoblotting |
| Biochem/physiol Actions: | IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. The antigen binding activity is mediated by the variable Fab region of IgG antibodies, whereas the effector activities are stimulated by the Fc domain. Anti-mouse IgG (Fc specific) antibody can be used to detect the optimal capture agents. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Immunogen: | Fc fragment of IgG purified from mouse. |
| Other Notes: | Antibody adsorbed with bovine, equine and human serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with bovine, horse or human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |