Monoclonal Anti-MDM2 antibody produced in mouse
SIGMA/M4308 - clone SMP14, ascites fluid
Synonym: Anti-ACTFS; Anti-HDMX; Anti-LSKB; Anti-hdm2
MDL Number: MFCD03792424
Product Type: Chemical
Immunoblotting Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 1:1,000 Monoclonal Anti-MDM2 Clone: SMP14 (Cat. No. M4308). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SMP14, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen ~90 kDa (additional band of approx. 55 kDa) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse (weak) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:1,000 using a whole extract of transfected 293T (human embryonal kidney) cells expressing human MDM2 | |
| UniProt accession no. | Q00987  |
| Application: | Monoclonal Anti-MDM2 antibody produced in mouse has been used in: • immunoblotting • immunoprecipitation (1 μg/ml) • fluorescence-activated cell sorting (FACS) • immunofluorescence (1:200) • immunohistochemistry • confocal immunofluorescence • immunocytochemistry • enzyme-linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Detects the MDM2 protein alone or in complex with E2F1, p53, and retinoblastoma proteins. A slight cross-reactivity is observed with cytokeratins 6, 14, and 16. |
| Biochem/physiol Actions: | Murine double minute (mdm2) mediates the transactivation of the tumor suppressor p53. MDM2 binds to the histone acetyltransferase p300 transcriptional coactivator/histone acetylase to mediate the degradation of p53. The nuclear localization signal of MDM2 mediates its shuttling from the nucleus to the cytoplasm and mediates p53 degradation. In addition to its relationships with p53, MDM2 activates cell proliferation by stimulating the S-phase-inducing transcription factors, E2F1/dimerization partner 1 (DP1). The amplification of the MDM2 gene is detected in the esophagogastric, liver, colon, breast and lung carcinomas. A disturbance in the optimal levels of the MDM2/p53 axis may have an impact on premature aging. Monoclonal antibody reacting specifically with MDM2 is an essential tool in defining the interactions and distributions of MDM2, its function in signaling pathways and mutual regulation of p53. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-MDM2 (mouse IgG1 isotype) is derived from the SMP14 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. The murine double minute (mdm2), an E3 ubiquitin ligase comprises an N-terminal p53 domain. The C-terminal domain encompasses the really interesting new gene (RING) domain, nuclear localization signal (NLS), nucleolar localization signal (NoLS) and a nuclear export signal (NES). MDM2 is mapped to the human chromosome 12q15. It is expressed as different splice variants. |
| Immunogen: | synthetic peptide corresponding to amino acids 154-167 of human MDM2 conjugated to KLH. |
| Physical form: | Monoclonal Anti-MDM2 is supplied as ascites fluid containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |