Anti-c-Myc antibody, Mouse monoclonal
SIGMA/M4439 - clone 9E10, purified from hybridoma cell culture
Synonym: Anti-c-Myc
Product Type: Chemical
Immunofluorescence HEK-293 cells overexpressing N-terminal c-Myc tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:2,000 Monoclonal Anti-c-Myc Clone: 9E10 (Cat. No. M4439). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence HEK-293 cells overexpressing C-terminal c-Myc tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:2,000 Monoclonal Anti-c-Myc Clone: 9E10 (Cat. No. M4439). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of E. coli cells expressing LacZ tagged with c-Myc were separated on SDS-PAGE and probed with Monoclonal Anti-c-Myc antibody produced in Mouse, Clone: 9E10 (Cat. No. M4439). The antibody was developed using 1:5,000 Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304). Lanes 1. 1:5,000 antibody 2. 1:7,000 antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 9E10, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 62 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1:5000 using c-myc tagged fusion protein | |
| UniProt accession no. | P01106  |
| Application: | Monoclonal Anti-c-Myc antibody produced in mouse is suitable for immunoprecipitation, immunoblotting, ELISA, immunofluorescence, microarray and electron microscopy. It has also been used in ChIP (Chromatin Immunoprecipitation). |
| Biochem/physiol Actions: | v-myc avian myelocytomatosis viral oncogene homolog belongs to MYC family. When overexpressed these proteins can cause DNA double-stand breaks (DSBs) and genome instability. This transcription factor MYC helps in regulating long noncoding RNAs (lncRNAs) which has been implicated in cancer cell proliferation and tumorigenesis. Its overexpression is also associated with various cancers, and in particular B cell lymphomas. Increased expression of c-MYC has a negative effect on the ability of B cell lymphomas to functionally present Ags/peptides to CD4+ T cells. This protein exerts suppressive effects at several critical checkpoints in Ag presentation. It is associated with Burkitt lymphoma and a group of diffuse large B-cell lymphoma (DLBCL). |
| General description: | c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper. Along with N-Myc and L-Myc, it forms the MYC proto-oncogene family. It forms a heterodimer with MAX, and this heterodimer binds to the E-boxes/CACGTG sequence motif on DNA. This gene is localized to human chromosome 8q24. |
| General description: | The mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc. |
| Immunogen: | synthetic peptide of the human p62c-Myc protein. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Working dilution: 1:5000 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |