Minimum Essential Medium Eagle

SIGMA/M4526 - Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Synonym: αMEM; EMEM; MEM

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-M4526-500ML	500 mL	$56.60 1/EAAdd To Favorites		Add To Favorites
45-M4526-6X500ML	500 mL	$317.00 6/EAAdd To Favorites		Add To Favorites
45-M4526-24X500ML	500 mL	$1040.00 24/EAAdd To Favorites		Add To Favorites

components	Earle’s salts (5% CO₂): yes sodium pyruvate: yes glucose: yes L-glutamine: no HEPES: no Hanks’ salts (2% CO₂): no phenol red: yes NaHCO₃: yes stable glutamine: no
form	liquid
impurities	endotoxin, tested
Quality Level	400
shipped in	ambient
sterility	sterile-filtered
storage temp.	2-8°C
technique(s)	cell culture \| mammalian: suitable

Application:	Minimum Essential Medium Eagle has been used to maintain: • immature cumulus-oocyte complexes from mice • human mesenchymal stem cells (MSC) • mouse pre-osteoblastic cell line
General description:	Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
General description:	This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.
Reconstitution:	Supplement with 0.292 g/L L-glutamine.