Monoclonal Anti-MAP1b antibody produced in mouse
SIGMA/M4528 - clone AA6, ascites fluid
Synonym: Anti-MAP1.2; Anti-MAP1X; Anti-MAP5
MDL Number: MFCD00164682
Product Type: Chemical
Immunofluorescence
Staining of polyester wax section through adult rat olfactory sensory epithelium using Monoclonal Anti-MAP1b, clone No. AA6 (Cat. No. M4528) showing intense reactivity in the olfactory nerve (red). Cell nuclei fluoresce blue due to Hoechst counterstain,
From Dr. A. Matus (Basel), reproduced from J. Neurosci., 1989; 9 (10). © 1989 The Society of Neuroscience. Used with permission.
Staining of polyester wax section through adult rat olfactory sensory epithelium using Monoclonal Anti-MAP1b, clone No. AA6 (Cat. No. M4528) showing intense reactivity in the olfactory nerve (red). Cell nuclei fluoresce blue due to Hoechst counterstain,
From Dr. A. Matus (Basel), reproduced from J. Neurosci., 1989; 9 (10). © 1989 The Society of Neuroscience. Used with permission.
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Monoclonal Anti-MAP1b antibody produced in Mouse, Clone: AA6 (Cat. No. M4528). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293). Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AA6, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, feline, bovine, hamster, chicken, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:500 using a fresh total rat brain extract or an enriched microtubule protein preparation | |
| UniProt accession no. | P46821  |
| Application: | Monoclonal Anti-MAP1b antibody produced in mouse has been used in: • immunohistochemistry • immunostaining • fluorescence microscopy |
| Biochem/physiol Actions: | Microtubule-associated protein (MAP1B) is the first MAP to appear in growing axons during development as it is present from the first emergence of the nascent axon from the cell body. Monoclonal Anti-MAP1b may be used to study MAP expression and cytological localization both in tissues and cell lines, under different developmental and environmental circumstances. |
| Biochem/physiol Actions: | Monoclonal Anti-MAP1b antibody is specific for MAP1b in humans, mice, rats, bovines, cats, hamsters and chickens. The antibody does not cross-react with other MAPs or tubulin. Addition of the antibody to microtubule proteins before polymerization results in abnormally short (but otherwise morphologically normal) microtubules. In immunohistochemical staining of brain tissue, the antibody shows selective labeling of neurons with stronger staining of axons, dendrites and cell bodies. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | MAP1b is a microtubule associated protein that interacts with actin filaments. MAP1b regulates microtubule stabilization and the development of axons. It may also act as a scaffolding protein Addition of antibody to microtubule proteins before polymerization in immunoassays results in abnormally short (but otherwise morphologically normal) microtubules. Immunohistochemical staining of brain tissue with the product shows selective labeling of dendritic trees throughout the brain. Monoclonal Anti-MAP1b does not react with tubulin of other microtubule associated proteins. |
| General description: | Monoclonal Anti-MAP1b (MAP5) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Microtubule-associated protein (MAP1B) is the major microtubule associated protein in developing brain which changes its expression during development. In the new born rat brain, it is a major component of microtubules but in the adult its level is ten-fold lower. |
| Immunogen: | rat brain microtubule-associated proteins (MAPs) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |