Mutanolysin from Streptomyces globisporus ATCCÂ 21553
SIGMA/M4782 - 0.2 μm filtered, lyophilized powder, ≥4000 units/mg protein (biuret)
CAS Number: 55466-22-3
MDL Number: MFCD00131635
Product Type: Chemical
Gram Positive Bacterial Cell Wall and Gram Negative Bacterial Cell Wall Mutanolysin lyses Listeria and other Gram-positive bacteria.
Structure of Lysing Enzymes - Mutanolysin and Lysozyme
| form | lyophilized powder |
| mol wt | 23 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥4000 units/mg protein (biuret) |
| sterility | 0.2 μm filtered |
| storage temp. | −20°C |
| Application: | Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA. |
| Biochem/physiol Actions: | Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N |
| Biochem/physiol Actions: | Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N |
| General description: | Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control. |
| Other Notes: | One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Physical form: | Lyophilized powder containing Ficoll and sodium succinate buffer salts |
| Preparation Note: | Prepared from M 9901 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4000 units/mg protein (biuret) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |