Anti-Myosin Va (LF-18) antibody produced in rabbit
SIGMA/M4812 - ~0.4 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-GS1; Anti-MYH12; Anti-MYO5; Anti-MYR12
MDL Number: MFCD03455613
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Rat cerebellum sections stained with 1:200 Anti-Myosin Va (LE-16) antibody produced in Rabbit (Cat. No. M5062) (A). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 1:200 Anti-Myosin Va (LF-18) antibody produced in Rabbit (Cat. No. M4812) (B). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-Myosin Va antibodies, M5062 (A) and M4812 (B), target different regions of Myosin Va show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat cerebellum sections with 1:200 Rabbit Anti-Myosin Va (LF-18) (Cat. No. M4812) using biotin/ExtrAvidin®-Peroxidase.
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Myosin Va (LF-18) antibody produced in Rabbit (Cat. No. M4812). The antibody was developed using 1:10,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~0.4 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 190 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | chicken, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections |
| microarray: suitable | |
| western blot: 1:1,000 using a rat brain extract | |
| UniProt accession no. | Q02440  |
| Application: | Anti-Myosin Va (LF-18) antibody produced in rabbit has been used in: • Western blot. • In situ proximity ligation assay (PLA). • Glutathione S-transferase (GST)-spinophilin co-sedimentation assay. |
| Biochem/physiol Actions: | Myosin 5a is an actin-based molecular motor responsible for transport of secretory granules in various cell systems, including insulin granule. Additionally, it also mediates the transport of cargo such as cell-surface receptors, pigment and organelles like endoplasmic reticulum to the plasma membrane. The encoded protein facilitates pigmentation and synaptic activity in the central nervous system. Genetic variations in the gene are associated with the development of immunological and neurological diseases, including Griscelli syndrome type 1 and Elejalde syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-myosin Va (LF-18) recognizes myosin Va (190kDa). Myosin 5a is encoded by the gene mapped to human chromosome 15q21.2. The encoded protein is a member of the unconventional myosin family. It is a two-headed motor containing two identical heavy chains and multiple light chains. Myosin Va is widely expressed, but at high levels in the brain, testes and melanocytes. |
| Immunogen: | synthetic peptide located near the C-terminus of chicken myosin Va (amino acids 1782-1799 with N-terminally added lysine) conjugated to KLH. This sequence is identical in human, mouse and rat. |
| Other Notes: | In order to obtain best results in different techniques and preparations we recommend determining optimal working dilutions by titration test. |
| Physical form: | Solution in 0.01 M phosphate buffered saline containing 1% BSA and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |