McCoy’s 5A Medium
SIGMA/M4892 - Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym: 5A basal medium
MDL Number: MFCD00217560
Product Type: Chemical
| components | phenol red: 0.011 g/L L-glutamine: 0.21915 g/L glucose: 3.0 g/L (Dextro) NaHCO3: no |
| form | powder |
| Quality Level | 500  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | McCoy′s 5A Medium has been used for culturing: • human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis • human colorectal HCT116 cells to study miR-224 over-expression in cancer • osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media |
| Biochem/physiol Actions: | McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types. |
| General description: | McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures. |
| Preparation Note: | Formulated to contain 11.9 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 2.2 g/L sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |