Medium 199
SIGMA/M5017 - With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym: Cell Culture Medium; M199 Medium; Medium 199 Solution; TCM 199
Product Type: Chemical
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| components | L-glutamine: yes phenol red: yes NaHCO3: no sodium pyruvate: no Earle’s salts (5% CO2): yes HEPES: no |
| form | powder |
| Quality Level | 500  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| General description: | Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues. |
| Preparation Note: | Formulated to contain 9.5 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 2.2 g/L sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |