Monoclonal Anti-5MTH Folic Acid antibody produced in mouse
SIGMA/M5028 - clone FA-24, ascites fluid
MDL Number: MFCD00164510
Product Type: Chemical
ELISA Monoclonal Anti-5MTH Folic Acid antibody produced in Mouse, Clone: FA-24 (Cat. No. M5028), followed by 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect 5MTH Folic Acid by indirect ELISA.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FA-24, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:5,000 |
| Application: | Monoclonal Anti-5MTH Folic Acid antibody is suitable for use in indirect (Enzyme Linked Immuno Sorbent Assay) ELISA (1:5,000) and competitive ELISA. |
| Application: | Monoclonal Anti-5MTH Folic Acid antibody is suitable for use in indirect ELISA (1:5,000). |
| Biochem/physiol Actions: | 5-Methyltetrahydrofolate (5-MTHF) is a cofactor that regulates the conversion of homocysteine to methionine in the presence of another co-factor, vitamin B12. 5-MTHF is also involved in modulating antioxidative and cognitive functions. Increased serum 5-MTHF levels have been linked to anemia and macrocytosis. The antibody reacts with 5MTHFA as a free reagent or when conjugated to carriers such as KLH or BSA. Furthermore, the product associates with 5MTHFA bound to the endogenous folate binder in human plasma and serum. The monoclonal antibody does not cross-react with tetrahydrofolic acid, folinic acid, dihydrofolic acid, and vitamin B12. |
| Biochem/physiol Actions: | Vitamin B12 and folic acid are metabolically interrelated. The enzyme methionine synthetase, which catalyzes the conversion of homocysteine to methionine, requires vitamin B12 and 5MTHFA as cofactors. In the absence of vitamin B12, 5MTHFA cannot be converted to tetra hydro folic acid and enter the metabolic pool of 1-carbon fragment acceptors. Vitamin B12 and folate deficiencies are the most common causes of megaloblastic anemia, abnormal hemopoiesis, interference in the maintenance of normal nerve tissue and general intracellular uptake and function disorders in humans. Folic acid deficiency is common in pregnant women, alcoholics, those whose diets do not include raw fruits and vegetables and people with structural or functional damage to the small intestine. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-5MTH Folic Acid antibody is specific for an epitope present on 5MTHFA and does not detect folic acid. This hybridoma is a cloned cell line derived from a fusion between a mouse myeloma cell line and splenocytes from BALB/c mice immunized with 5MTH Folic acid conjugated to KLH. |
| Immunogen: | 5MTH folic acid, conjugated to KLH. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |