MES sodium salt
SIGMA/M5057 - BioReagent, ≥99% (titration), crystalline
Synonym: 2-
CAS Number: 71119-23-8
Empirical Formula (Hill Notation): C6H12NNaO4S
Molecular Weight: 217.22
EC Number: 275-203-2
MDL Number: MFCD00065473
Linear Formula: C6H12NNaO4S
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M5057-25G
This image depicts SKU: M5057-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M5057-10G
This image depicts SKU: M5057-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M5057-100G
This image depicts SKU: M5057-100G
| application(s) | diagnostic assay manufacturing |
| assay | ≥99% (titration) |
| form | crystalline |
| InChI | 1S/C6H13NO4S.Na/c8-12(9,1 |
| InChI key | IRHWMYKYLWNHTL-UHFFFAOYSA |
| pH | 8.9-10.1 (1% in water) |
| pKa | 6.1 |
| product line | BioReagent |
| Quality Level | 400  |
| SMILES string | [Na+].[O-]S(=O)(=O)CCN1CC |
| solubility | water: 0.5 g/mL, clear, colorless |
| storage temp. | room temp |
| useful pH range | 5.5-6.7 |
| Application: | MES sodium salt has been used: • To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNA • To equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization |
| General description: | MES is one of the so-called "Good" buffers developed for biological applications. This product is tested for trace ions. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±-1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published. |
| Other Notes: | Easily compare specifications for MES sodium salt products with the MES sodium salt specification table . |
| Packaging: | 10, 25, 100 g in poly bottle |
| Preparation Note: | MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions should be stable at 2-8°C for months. Sterilization should be by filteration through 0.2μm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (titration) |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |