Anti-Myosin Va (LE-16) antibody produced in rabbit
SIGMA/M5062 - ~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD03455612
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Rat cerebellum sections stained with 1:200 Anti-Myosin Va (LE-16) antibody produced in Rabbit (Cat. No. M5062) (A). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 1:200 Anti-Myosin Va (LF-18) antibody produced in Rabbit (Cat. No. M4812) (B). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-Myosin Va antibodies, M5062 (A) and M4812 (B), target different regions of Myosin Va show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin fixed, paraffin-embedded Rat cerebellum tissue sections were stained with 1:200 Anti-Myosin Va (LE-16) antibody produced in Rabbit (Cat. No. M5062) followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Myosin Va (LE-16) antibody produced in Rabbit (Cat. No. M5062). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:1,000 antibody 2. 1:500 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~0.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 190 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections |
| microarray: suitable | |
| western blot: 1:500 using a rat brain extract | |
| UniProt accession no. | Q02440  |
| Application: | Anti-Myosin Va (LE-16) antibody produced in rabbit is used in immunoblotting and immunohistochemistry. |
| Biochem/physiol Actions: | Myosin Va is implicated in the regulation of vesicle trafficking in neurons and melanocytes. It regulates melanosome distribution along microfilaments. It is found in association with the centrosome at all stages of the cell cycle. In the interphase stage, myosin Va is found in pericentriolar material. During cell division, it is found in the cytoplasm and concentrates in a trail between migrating centrioles and in the mitotic spindle poles and spindle fibers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myosin Va (p190) is a member of the unconventional class of myosins, distinct from both the myosins I and myosin II. It is present in neuronal and non neuronal cells of the brain. Class V myosins are widely expressed actin-based motors. Class V myosins have two motor head domains typical of myosins, and an extended regulatory neck domain with six tandem IQ domains that bind multiple calmodulin light chains. In addition, myosin V contains a unique 400 amino acids globular tail domain that may direct myosin V to its target or determine the cargo to which it binds. |
| Immunogen: | synthetic peptide located near the C-terminus of chicken myosin Va (amino acids 1705-1720 with N-terminally added lysine) conjugated to KLH. This sequence is identical in human, mouse and rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |