Anti-Myosin VI (KA-15) antibody produced in rabbit
SIGMA/M5187 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DFNA22; Anti-DFNB37
MDL Number: MFCD03455614
Product Type: Chemical
Immunofluorescence Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:75 Anti-Myosin VI (KA-15) antibody produced in Rabbit (Cat. No. M5187). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Immunoblotting Madin Darby Canine Kidney (MDCK) was separated on SDS-PAGE and probed with AIA TO MYOSIN VI (KA-15) (RB) (Cat. No. M5187). The antibody was developed with Alk Phos APA Rabbit IgG (gt) (Cat. No. A9919) and a NBT/BCIP substrate. Antibody dilution 1/2,000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~150 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:75 using cultured rat NRK cells |
| microarray: suitable | |
| western blot: 1:1,000 using a whole extract of cultured dog MDCK cells |
| Application: | Anti-Myosin VI (KA-15) antibody has been used: • in immunoblotting • in immunocytochemistry • in immunofluorescence • immunohistochemistry • proximity ligation assay |
| Biochem/physiol Actions: | Myosin VI participates in the generation of cell shape change, cell motility, membrane remodeling, and possibly in organelle and particle transport or tethering. It is also involved in membrane trafficking pathways in cultured mammalian cells where it is associated with the membrane ruffles and the trans-Golgi network. The unusual direction of Myosin VI movement may suggest that it brings materials or membranes into the cell. Its activity in tissue cultured cells is thought to be regulated by phosphorylation. A mutation in Myosin VI was described recently in human autosomal dominant non syndromic hearing loss. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myosin VI (MYO6) is localized to the Golgi complex and is expressed in the hair cells of the ear. It is a two-headed myosin with a short coiled-coil segment in its tail. The motor domain of MYO6 has two insertions. The gene encoding this protein is localized on chromosome 6q13. |
| General description: | Myosin VI is a relatively abundant widespread unconventional myosin composed of an N-terminal motor domain, a light chain binding neck region, a coiled-coiled region, and a highly conserved C-terminal domain. At the ′converter′ region, between the catalytic head and the neck region of Myosin VI, there is a characteristic linker about 50 amino acids long. Native Myosin VI is apparently a two-headed dimer of the heavy chains with each heavy chain bound to calmodulin light chain. |
| Immunogen: | synthetic peptide corresponding to an epitope within the C-terminal of human Myosin VI, with N-terminal cysteine added, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |