MES hydrate
SIGMA/M5287 - ≥99.5% (titration), pH 2.5-4.0 (0.5 M in H2O), BioXtra
Synonym: 2-
CAS Number: 1266615-59-1
Empirical Formula (Hill Notation): C6H13NO4S · xH2O
Molecular Weight: 195.24 (anhydrous basis)
MDL Number: MFCD00149409
Linear Formula: C6H13NO4S · xH2O
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M5287-50G
This image depicts SKU: M5287-50G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M5287-250G
This image depicts SKU: M5287-250G
| absorption | ≤0.020 at 280 in H2O at 0.5 M |
| ≤0.025 at 260 in H2O at 0.5 M | |
| anion traces | chloride (Cl-): ≤0.005% |
| application(s) | cell analysis life science and biopharma sample preparation |
| assay | ≥99.5% (titration) |
| cation traces | Al: ≤0.0005% |
| As: ≤0.0001% | |
| Ba: ≤0.0005% | |
| Bi: ≤0.0005% | |
| Ca: ≤0.002% | |
| Cd: ≤0.0005% | |
| Co: ≤0.0005% | |
| Cr: ≤0.0005% | |
| Cu: ≤0.0005% | |
| Fe: ≤0.0005% | |
| K: ≤0.005% | |
| Li: ≤0.0005% | |
| Mg: ≤0.0005% | |
| Mn: ≤0.0005% | |
| Mo: ≤0.0005% | |
| Na: ≤0.005% | |
| Ni: ≤0.0005% | |
| Pb: ≤0.0005% | |
| Sr: ≤0.0005% | |
| Zn: ≤0.0005% | |
| color | white |
| form | crystalline powder |
| ign. residue (900°C) | ≤0.05% |
| impurities | Insoluble matter, passes filter test |
| InChI | 1S/C6H13NO4S.H2O/c8-12(9, |
| InChI key | MIIIXQJBDGSIKL-UHFFFAOYSA |
| pH | 2.5-4.0 (0.5 M in H2O) |
| pKa | 6.1 |
| product line | BioXtra |
| Quality Level | 400  |
| SMILES string | O.OS(=O)(=O)CCN1CCOCC1 |
| solubility | H2O: 0.5 M, clear, colorless |
| water: 335.3 g/L at 20 °C | |
| storage condition | dry at room temperature |
| storage temp. | room temp |
| useful pH range | 5.5-6.7 |
| Application: | MES Hydrate has been used: • As a component of extraction buffer in the extraction of the enzyme BACE1 • As a component of the reaction mixture for the measurement of 5,10-methenyltetrahydrofo • As a component of MES coupling buffer during the coupling of mAb or GST-fusion proteins to carboxylate-modified polystyrene latex (CML) beads • to adjust the pH of the solution for the fluorescent labeling • as a component of electrophoresis buffer |
| Features and Benefits: | • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C) • Highly soluble in water • Minimal metal ion binding • Less toxic to cells than other buffers such as Tris and phosphate • Stable in a wide pH range • Low UV absorptivity • Minimal reactivity |
| General description: | MES hydrate buffer (2-(N-morpholino)ethanesu MES hydrate buffer also exhibits high water solubility and minimal metal ion binding, making it an ideal choice for various applications: • Cell Biology: MES hydrate buffer is often the preferred choice for buffering cell culture media due to its lower toxicity to cells compared to other buffers like Tris and phosphate. • Protein Purification and Extraction: Its stability across a wide pH range and minimal metal ion binding make MES hydrate buffer a valuable asset in protein purification and extraction protocols. • Gel Electrophoresis: MES hydrate buffer is also commonly used as a running buffer in denaturing gel electrophoresis, ensuring reliable results in protein separation and analysis. |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | 50, 250 g in poly bottle |
| Preparation Note: | A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered. |
| Preparation Note: | Solutions are stable at 2-8 C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.5% (titration) |
| Storage Temp. | room temp |
| UNSPSC | 12352106 |