Murashige and Skoog Basal Salt Mixture (MS)
SIGMA/M5524 - powder, suitable for plant cell culture
Synonym: MS Basal Salts; MS0 Basal Salts; MSO Basal Salts
Product Type: Chemical
Murashige and Skoog Basal Salt Mixture (MS) This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
| application(s) | agriculture |
| form | powder |
| Quality Level | 100  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | plant: suitable |
| Application: | Murashige and Skoog Basal Salt Mixture (MS) has been used to support the growth and regeneration of transformed tobacco cells. It has also been used as a growth medium for germination of Arabidopsis thaliana (Col-0) seeds. |
| General description: | Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and Kinetin (cytokinin/cell division promoter). |
| Other Notes: | Storage Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening. Store dry medium in a desiccator at 0-5 °C. Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used. Media Preparation |
| Preparation Note: | Formulated to contain 4.3 grams of powder per liter of medium. |
| Preparation Note: | Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg’s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture. |
| Preparation Note: | With the macro- and micronutrients as described by Murashige and Skoog (1962). Media Formulation |
| Symbol |   GHS03,GHS07 |
| Signal word | Warning |
| Hazard statements | H272 - H319 |
| Precautionary statements | P210 - P220 - P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| Hazard Codes | O,Xi |
| Risk Statements | 8-36/37/38 |
| Safety Statements | 26 |
| RIDADR | UN 1477 5.1 / PGII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |