Thiazolyl Blue Tetrazolium Bromide

SIGMA/M5655 - powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

Synonym: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; MTT; Methylthiazolyldiphenyl-tetrazolium bromide

CAS Number: 298-93-1
Empirical Formula (Hill Notation): C₁₈H₁₆BrN₅S
Molecular Weight: 414.32
EC Number: 206-069-5
MDL Number: MFCD00011964
Linear Formula: C₁₈H₁₆BrN₅S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-M5655-100MG	100 mg	$52.10 1/EA	Add To Favorites
45-M5655-500MG	500 mg	$120.00 1/EA	Add To Favorites
45-M5655-1G	1 g	$209.00 1/EA	Add To Favorites
45-M5655-5X1G	1 g	$872.00 5/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M5655-100MG.

Properties
Descriptions
Safety Info.
Basic Data

assay	≥97.5% (HPLC)
form	powder
InChI	1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
InChI key	AZKSAVLVSZKNRD-UHFFFAOYSA-M
mp	195 °C (dec.) (lit.)
product line	BioReagent
Quality Level	200
SMILES string	[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
solubility	H₂O: 5 mg/mL
storage temp.	2-8°C
technique(s)	cell culture \| insect: suitable
	cell culture \| mammalian: suitable

Application:	Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Application:	Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Application:	Thiazolyl Blue Tetrazolium Bromide has been used as a colorimetric metabolic activity indicator in cell viability assays (MTT assay). It has also been used to determine cell proliferation.
Other Notes:	2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Packaging:	1 g in glass bottle
Packaging:	100, 500 mg in glass insert
Packaging:	15 mg in poly bottle

Symbol	GHS07,GHS08
Signal word	Warning
Hazard statements	H315 - H319 - H335 - H341
Precautionary statements	P201 - P202 - P261 - P302 + P352 - P305 + P351 + P338 - P308 + P313
Hazard Codes	Xn
Risk Statements	36/37/38-68
Safety Statements	26-36/37
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥97.5% (HPLC)
mp	195 °C (dec.) (lit.)
Storage Temp.	2-8°C
UNSPSC	12352207

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