Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit
SIGMA/M5670 - whole antiserum
Synonym: Anti-MAP Kinase (ERK-1, ERK-2); Anti-Mitogen Activated Protein Kinase
MDL Number: MFCD00282359
Product Type: Chemical
Western Blotting Cell line lysates were separated on SDS-PAGE and probed with 1:20,000 Rabbit Anti-MAP Kinase (ERK-1, ERK-2) (Cat. No. M5670). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. K562; 2. COS-7; 3. HL-60; 4. RAW-264; 5. A431; 6. HeLa; 7. C2C12; 8. JURKAT; 9. HEK-293; 10. HepG2; 11. NIH-3T3; 12. Rat Brain
Immunoprecipitation Rabbit Anti-MAP Kinase (ERK-1, ERK-2) (Cat. No. M5670) was used to immunoprecipitate MAPK1/MAPK3 from HeLa whole cell lysate. Detection antibody: Rabbit Anti-MAP Kinase (ERK-1, ERK-2) (Cat. No. M5670) followed by Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 5 μL antibody 2. Negative control: 10 μg nonrelevant antibody 3. Positive control: HeLa whole cell lysate
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| mol wt | antigen ERK-2 42 kDa |
| antigen, ERK-1 44 kDa | |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:40,000-1:20,000 using rat brain extract and mouse NIH 3T3 fibroblast extract, respectively | |
| UniProt accession no. | P28482  |
| Application: | Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit has been used in western blot analysis at a minimum dilution of 1:40000 in rat brain extract and at 1:20000 in mouse NIH 3T3 fibroblasts. |
| Biochem/physiol Actions: | Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival. MAPK plays a crucial role in various signal transduction pathways, leading signals of growth factor and G protein coupled receptors to their intracellular targets. MAP kinase regulates several cellular processes including proliferation, differentiatio, cellular morphology and oncogenesis. |
| Biochem/physiol Actions: | Staining of the 42 kDa and 44 kDa bands is specifically inhibited with MAP kinase peptide (317-339), but not with MAP kinase kinase (MEK) peptide (34-48) corresponding to N-terminal sequence of MEK. Anti-MAPK Kinase (ERK-1, ERK-2) specifically reacts with both phosphorylated and unphosphorylated forms of ERK-1 and ERK-2 (44 and 42 kDa, respectively). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | MAP kinase (MAPK, mitogen-activated protein kinase) is also termed as extracellular regulated protein kinase (ERK). It consists of a family of protein kinases. MAP kinase isoforms are widely expressed in the central nervous system, thymus, spleen, heart, lung, kidney, and in high levels in PC12 cells and in fibroblasts. |
| Immunogen: | synthetic peptide corresponding to amino acids 317-339 derived from subdomain XI of human MAP kinase (ERK1). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |