Anti-MAP Kinase Kinase (MEK, MAPKK) antibody produced in rabbit
SIGMA/M5795 - whole antiserum
Synonym: Anti-MAPKK; Anti-MEK
MDL Number: MFCD00239713
Product Type: Chemical
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-MAP Kinase Kinase (MEK, MAPKK) antibody produced in Rabbit (Cat. No. M5795). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:20,000 antibody 2. 1:40,000 antibody 3. Negative Control
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| mol wt | antigen 45 kDa (MEK1a) |
| antigen 46 kDa (MEK2) | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:20,000 using rat brain extract and mouse NIH 3T3 fibroblast lysate | |
| UniProt accession no. | P36507  |
| Application: | Anti-MAP Kinase Kinase (MEK, MAPKK) antibody produced in rabbit has been used in immunoblotting and for analysis of signaling pathways. |
| Biochem/physiol Actions: | Antibodies that react specifically with MEK may be used to study the specific activation requirements, differential tissue expression and intracellular localization of MEK in normal and neoplastic tissue. |
| Biochem/physiol Actions: | MAP Kinase Kinase (MAPKK, MEK1 and MEK2) belong to a family of enzymes that activates their substrate by dual phosphorylation at threonine and tyrosine residues. MEK1 and MEK2 have 86% homology in their catalytic domain. Activation of MEK1/2 is mediated in mitogen-stimulated cells by Raf-1 kinase. MEK1/2 then activates ERK1/2 leading to the activation of several downstream targets that contribute to cell proliferation, apoptosis and motility. In addition to ERK1/2, there may be non-catalytic effectors of MEK1/2. MEK isoforms appear to be widely expressed in the central nervous system, thymus, spleen, heart, lung, and kidney. Active forms of MEK1/2 are sufficient for the transformation of NIH3T3 cells of the differentiation of PC-12 cells. |
| Biochem/physiol Actions: | Reacts with MEK-1a and MEK-2. Staining of MEK-1a and MEK-2 is specifically inhibited with MEK-1 peptide (34-48), but not with MAP kinase peptide (317-399) corresponding to subdomain XI of ERK1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | MAP kinase kinase (MAPKK, MEK) consists of three different isoforms with a high degree of homology between them, MEK-1a (45 kDa), MEK-1b (41 kDa), and MEK-2 (46 kDa). MEK isoforms are generally expressed, in the central nervous system, thymus, spleen, heart, lung, kidney. In addition, it is also expressed at high levels in PC12 cells and in fibroblasts. |
| Immunogen: | synthetic peptide corresponding to amino acids 34-48 of human MAP kinase kinase 1 (MEK-1). |
| WGK Germany | 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |